Speck formation assay
WebApr 19, 2024 · CTB assay was performed on the remaining living cells following the manufacturer's recommendations. For activation of NLRP3 with MSU crystals, ... Hoss F, Rolfes V, Davanso MR, Braga TT, Franklin BS (2024) Detection of ASC speck formation by flow cytometry and chemical cross-linking. In Innate Immune Activation, D De Nardo, ... WebFeb 25, 2024 · Apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) is an adaptor protein that is essential for the activation of several …
Speck formation assay
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WebHence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome activation by ASC … WebSep 27, 2012 · Speck Quantification Assay HEK293 cells were transfected with ASC-myc and positively selected using 1 mg/ml G418 for 4 weeks. HEK293-ASC cells (1 × 10 5) were co-transfected with 250 ng of each NLRP3 expression plasmid and pyrin expression plasmid using Lipofectamine LTX (Invitrogen) according to the manufacturer’s instructions.
WebSep 14, 2024 · ASC Speck formation. One of the possible projects is understanding how protein sequence variants impact inflammasome formation. There are multiple possible … WebFree ASC and proinflammatory cytokine levels were determined by ELISA and multiplex assay. ... except the CAPS patients. Following inflammasome activation and ASC speck …
WebMethods: Peripheral blood of JIA patients ( n = 46) was investigated for ASC speck formation, a key step in inflammasome activation, by flow cytometry and immunofluorescence. Free ASC and proinflammatory cytokine levels were determined by ELISA and multiplex assay. Webspeck formation, BMMs were stimulated with RANKL in the presence or absence of YCG063, stained with an anti-ASC antibody, and Alexa 488-conjugated second antibody. To further analyze the underlying mechanisms, the cytotoxicity assay was performed in RANKL-treated BMMs in the presence or absence of LPS with or without MCC950 or Z-YVAD-fmk. The
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WebJan 1, 2013 · Hence, ASC speck formation can be used as a simple upstream readout for inflammasome activation. Here, we describe a method for analyzing inflammasome … the fuehrer\\u0027sWebInflammasome assembly results in the formation of a large intracellular protein scaffold driven by the oligomerization of the adaptor protein apoptosis-associated speck-like … the air lineWebStutz A, Horvath GL, Monks BG, Latz E (2013) ASC speck formation as a readout for inflammasome activation. Methods Mol Biol 1040:91–101 Fernandes-Alnemri T, Alnemri ES (2008) Assembly, purification, and assay of the activity of the ASC pyroptosome. Methods Enzymol 442:251–270 the fud trailer floridaWebDec 13, 2024 · After 24 h, HEK293 cells were harvested to analyze the speck formation by flow cytometry and confocal microscopy as well as the oligomerization formation by … thefudtrailer.comWebFeb 4, 2024 · ASC-Speck Formation Among Blood Leukocytes of Sepsis Patients After inflammasome activation, ASC assembles to a protein complex, termed “speck”. The … theairlinedesk incWebRockefeller University Press the fuehrer\\u0027s face songWebThis study aimed to establish and validate a new functional assay to evaluate the pathogenicity of MEFV variants. Methods: ... Cell death was caspase-1 dependent and was accompanied by ASC speck formation and IL-1β secretion, indicating that pathogenic MEFV variants induced abnormal pyrin inflammasome activation and subsequent pyroptotic cell ... the fudgies group