Phis2.1 载体图谱

Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … WebbVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited at Addgene are available for purchase through this website.

pHIS2说明书-分析方法-生物在线 Lab-on-Web

WebbDifferent pairs of plasmids (pGAD-OsWRKY54 and pHIS2- ProOsHKT1;5 , or control pGADT7 and pHIS2) were transformed into yeast cells. Transformed cells were spotted on SD medium containing 0, 50, or 75 mM 3-amino-1,2,4-triazole at 30 °C for 3 … WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... graham norton show wikipedia https://e-shikibu.com

T-Vector pMD19 (Simple) Sequence and Map - SnapGene

http://www.youbio.cn/product/vt1637 http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 Webb1 sep. 2000 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs (protein cloned in ... china high blood pressure medication

pHis2酵母单杂交载体 631903报价/价格pHis2酵母单杂交载体质粒 …

Category:酵母杂交实验常见问题——单杂知识及自激活检测篇_元件_-bait_作用

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Phis2.1 载体图谱

Addgene: Vector Database - pHIS-2

WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 WebbpHISi载体价格3000元，pHISi载体质粒图谱(Vector map)，载体序列(Sequence)见下文。

Phis2.1 载体图谱

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WebbpHIS2.1 BioVector NTCC质粒载体菌种细胞基因保藏中心. 价 格： ¥8910. 货 号： pHIS2.1. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！. http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf

WebbT-Vector pMD19 (Simple) Linearized vector with no MCS and with a single 3'-T at each end, for TA cloning of PCR products and blue-white screening. Explore Over 2.7k Plasmids: TA and GC Cloning Vectors More Plasmid Sets. WebbpHISi is a yeast integration and reporter vector for use with the MATCHMAKER One-Hybrid System. pHISi contains the yeast HIS3 gene downstream of the MCS and the minimal …

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb16 mars 2024 · 诱饵载体：pHis2-DNA（筛选标记Trp报告基因His） 文库载体：pGADT7-cDNA（筛选标记Leu） 实验步骤： 一、转化诱饵载体（参考SK2400中质粒转化步骤） 1. YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2. 制备感受态并将pHis2-DNA转化进入感受态细胞； 3. SD/-Trp平板检测转化结果。 二、自 …

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Webb19 nov. 2012 · 我把要验证的转录因子构建到pGADT7-rec2载体上，与pHis2的空载共同转化Y187，本来要拿这个做阴性对照的，可是在三缺板子上长得很欢乐呀，加了3AT，并且 … graham norton show will smith and jaden smithhttp://muchong.com/t-9775583-1 graham norton show youtube 2020WebbpHis2载体价格650元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体。 china high dog bowlsWebb质粒名称：pHis2. 出品公司：Clontech . 质粒类型： 酵母单杂交系统. 克隆方法： 多克隆位点，限制性内切酶. 载体大小： 7207bp . 原核抗性： Kanamycin (卡那霉素) 真核抗 … graham norton show with jason momoaWebb1 juni 2024 · Digest the pHIS2 vector with Sma I purify it by agarose gel electrophoresis (0.8% agarose). 5. For addition of a single ‘T’ base at the ends of the linearized pHIS2 plasmid, prepare the following mix in a 10 μL total volume: 1 μg pHIS2 ( Sma I linearized), 0.5 μL dTTP (stock concentration 10 mM), 1 μL 10× ExTaq buffer, 0.5 U ExTaq. china high blood prWebb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … graham norton s husbandhttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html graham norton show with sarah millican